CAS NO 2869-83-2 Janus Green B Powder

Product Name:

Janus Green B

Synonyms:

Janus green B (CI x 11050);Janus Green B,pure,high purity biological stain;Janu;Janus Green B, high purity biological stain;Janus Green B, high purity biological stain, pure 5GR;JANUS GREEN (C.I. 11050) FOR MICROSCOPY;Janus Green B, Pure;Janus Green B chloride

CAS:

2869-83-2

MF:

C30H31ClN6

MW:

511.07

EINECS:

220-695-6

Product Categories:

Phenazine;Organics

Mol File:

2869-83-2.mol

Melting point

160 °C (dec.)(lit.)

vapor pressure

2.6 mm Hg ( 20 °C)

solubility

H2O: 1 M hot, clear, colorless

form

Powder

Colour Index

11050

color

≤10(APHA)

Water Solubility

Soluble in water (20 mg/ml at 20°C), ethanol, and slightly soluble in alcohol.

λmax

660nm, 395nm

Merck

14,5255

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Antimalarialagents; diagnosis of diseases related to amyloid accumulation; diagnostic assays; detecting fungi; nucleic acids; sugars

InChIKey

XXACTDWGHQXLGW-UHFFFAOYSA-M

CAS DataBase Reference

2869-83-2(CAS DataBase Reference)

EPA Substance Registry System

Phenazinium, 3-(diethylamino)-7-[[4-(dimethylamino)phenyl]azo]-5-phenyl-, chloride (2869-83-2)

Chemical Properties

green powder

Uses

As dye for cotton, wool; as biological stain; in electrodeposition of copper, Brown, Fellows, US 2882209 (1959 to Udylite Res. Corp.).

Uses

Janus Green B is a stain that interacts with DNA and has been used for histology studies including mitochondrial staining, in which its oxidation and reduction reveals alterations to the electron transfer chain. The stain has been utilized in studies for effective and rapid staining of insect chordotonal organs and peripheral nerves, where the nerves appear dark blue in comparison to non stained tissue. With the use of Janus Green B, rabbit lymphatic vessel studies demonstrate that threadlike structures within the lymphatic vessels, known as Bonghan ducts, contain a high density of mitochondria in their cells.

Definition

ChEBI: Janus Green B chloride is an organic chloride salt. It has a role as a dye. It contains a Janus Green B cation.

Safety Profile

Poison by intraperitoneal and intravenous routes. Moderately toxic by ingestion, skin contact, subcutaneous, and intramuscular routes. Human systemic effects by inhalation: nausea or vomiting. A skin and severe eye irritant. Flammable liquid when exposed to heat or flame; can react with oxidizing materials. To fight fire, use alcohol foam, CO2, dry chemical. When heated to decomposition it emits toxic fumes of NOx. See also AMINES.

Purification Methods

The dye dissolves in H2O to give a bluish violet solution which becomes colourless when made 10M in NaOH. It dissolves in EtOH to give a blue-violet colour, filter from insoluble material, then add dry Et2O whereby the dye separates out leaving a small amount of blue colour in solution. Filter off the solid and dry it in a vacuum. Store it in a dark bottle. [Colour Index Vol 4, 3rd edn, 4015 1971.]