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Product Details:
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| Name: | 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic Acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic Acid) | Cas No: | 7365-45-9 |
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| Application: | Molecular Biology | Purity: | 99% |
| Classification: | Biological Buffer | Form: | Crystal Powder |
| Color: | White | Samples: | Available |
| Package: | 1kg/5kg/25kg | Transport: | Courier/Air/Sea |
| High Light: | HEPES Biochemical Reagents,ISO9001 Biochemical Reagents,CAS 7365-45-9 |
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HEPES CAS 7365-45-9 Biological Buffers Molecular Biology Grade (free acid)
| HEPES Basic information |
| Product Name: | HEPES |
| Synonyms: | HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE;HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+%;N-(2-HYDROXYETHYL)-PIPERAZINE-N'-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID));Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz;1-HeptaneSulfonicAcidSod.SaltGr;N-(2-Hydroxyethyl)Piperazine-N'-(3-EthanesulfonicAcid);N-(2-Hydroxyethyl)Piperazine-N'-(2-EthanesulfonicAcid)(Hepes);Hepes,LowSodium,FreeAcid |
| CAS: | 7365-45-9 |
| MF: | C8H18N2O4S |
| MW: | 238.3 |
| EINECS: | 230-907-9 |
| Product Categories: | Disinfectants;Biochemistry;Good's Buffers;Buffer;ReagentsBiological Buffers;GMP;AlphabeticalMolecular Biology;Biological Buffers;BioUltra Buffers;BioUltraMolecular Biology;DNA&RNA Purification;Molecular Biology Reagents;Zwitterionic;VX:15689727968 |
| Mol File: | 7365-45-9.mol |
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| HEPES Chemical Properties |
| Melting point | 234-238 °C |
| density | 1.07 g/mL at 20 °C |
| refractive index | n |
| storage temp. | 2-8°C |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | powder |
| color | White |
| PH | 5.0-6.5 (1Maqueous solution) |
| PH Range | 7.0 - 7.6 |
| pka | 7.5(at 25℃) |
| Water Solubility | Soluble |
| λmax | λ: 260 nm Amax: 0.06 λ: 280 nm Amax: 0.04 |
| Merck | 14,4654 |
| BRN | 883043 |
| Stability: | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
| HEPES Usage And Synthesis |
| Description | HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. |
| Chemical Properties | White crystalline powder |
| Uses | Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability. |
| Uses | Biological buffer. |
| Uses | HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research. HEPES has been used as a component of: Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices. Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity. |
| Definition | ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2. |
| General Description | HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions. |
| Biological Activity | HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media. |
| Biological Activity | Multi purpose buffer used in biological research. |
| Purification Methods | Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.] |
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