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Product Details:
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Product Name: | Janus Green B | CAS NO: | 2869-83-2 |
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Form: | Powder | Sample: | Available |
Formula: | C30H31ClN6 | Composition: | Dye Content, 65% |
Stability: | Stable. Incompatible With Strong Oxidizing Agents. | Water Solubility: | Soluble In Water (20 Mg/ml At 20°C), Ethanol, And Slightly Soluble In Alcohol. |
High Light: | Janus Green B Biological Stains,2869-83-2 Biological Stains,65% Janus Green B |
CAS NO 2869-83-2 Janus Green B Dye content 65 %
Product Name: |
Janus Green B |
Synonyms: |
Janus green B (CI x 11050);Janus Green B,pure,high purity biological stain;Janu;Janus Green B, high purity biological stain;Janus Green B, high purity biological stain, pure 5GR;JANUS GREEN (C.I. 11050) FOR MICROSCOPY;Janus Green B, Pure;Janus Green B chloride |
CAS: |
2869-83-2 |
MF: |
C30H31ClN6 |
MW: |
511.07 |
EINECS: |
220-695-6 |
Product Categories: |
Phenazine;Organics |
Mol File: |
2869-83-2.mol |
Chemical Properties |
green powder |
Uses |
As dye for cotton, wool; as biological stain; in electrodeposition of copper, Brown, Fellows, US 2882209 (1959 to Udylite Res. Corp.). |
Uses |
Janus Green B is a stain that interacts with DNA and has been used for histology studies including mitochondrial staining, in which its oxidation and reduction reveals alterations to the electron transfer chain. The stain has been utilized in studies for effective and rapid staining of insect chordotonal organs and peripheral nerves, where the nerves appear dark blue in comparison to non stained tissue. With the use of Janus Green B, rabbit lymphatic vessel studies demonstrate that threadlike structures within the lymphatic vessels, known as Bonghan ducts, contain a high density of mitochondria in their cells. |
Definition |
ChEBI: Janus Green B chloride is an organic chloride salt. It has a role as a dye. It contains a Janus Green B cation. |
Safety Profile |
Poison by intraperitoneal and intravenous routes. Moderately toxic by ingestion, skin contact, subcutaneous, and intramuscular routes. Human systemic effects by inhalation: nausea or vomiting. A skin and severe eye irritant. Flammable liquid when exposed to heat or flame; can react with oxidizing materials. To fight fire, use alcohol foam, CO2, dry chemical. When heated to decomposition it emits toxic fumes of NOx. See also AMINES. |
Purification Methods |
The dye dissolves in H2O to give a bluish violet solution which becomes colourless when made 10M in NaOH. It dissolves in EtOH to give a blue-violet colour, filter from insoluble material, then add dry Et2O whereby the dye separates out leaving a small amount of blue colour in solution. Filter off the solid and dry it in a vacuum. Store it in a dark bottle. [Colour Index Vol 4, 3rd edn, 4015 1971.] |
Application
Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.
Biochem/physiol Actions
Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomolecules, nucleic acids and chromosomes. In addition, it is also used to stain various living organisms including fungi, yeast cell, brain, spinal cord, sperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent. It also facilitates diagnostic assays and diagnosis of disease related to amyloid accumulation.
FAQ
1:Can I get some samples before bulk order?
A: Most products provide free samples, but the shipping cost be paid by customers.
2: What's your MOQ?
A: We can have a discussion.
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5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.
6:How do you treat quality complaint?
A: First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.
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