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CAS NO 314-13-6 Evans Blue powder

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CAS NO 314-13-6 Evans Blue powder

CAS NO 314-13-6 Evans Blue powder
CAS NO 314-13-6 Evans Blue powder CAS NO 314-13-6 Evans Blue powder

Large Image :  CAS NO 314-13-6 Evans Blue powder

Product Details:
Place of Origin: China
Brand Name: YunbangPharm
Certification: ISO9001
Model Number: YB011
Payment & Shipping Terms:
Minimum Order Quantity: Negotiable
Price: Negotiable
Packaging Details: 100g,200g,1kg/Bottle, 5-25kg/Drum
Delivery Time: 3-5 working days
Payment Terms: L/C, D/A, D/P, T/T, Western Union

CAS NO 314-13-6 Evans Blue powder

Product Name: Evans Blue CAS NO: 314-13-6
Form: Powder Sample: Available
Formula: C34H29N6NaO14S4 Colour: Dark Brown
Storage Condition: Room Temp Stability: DMSO (Slightly), Water (Slightly)
High Light:

CAS 314-13-6 Direct Blue


Direct Blue Biochemical Reagents


ISO9001 Direct Blue Powder

CAS NO 314-13-6 Evans Blue powder


Product Name:

Evans Blue









Product Categories:

MATULANE;Biological Dye;Organics;Glutamate receptor

Mol File:


Direct Blue 53 Structure

Chemical Properties

brownish crystalline powder




Evans Blue is used for estimation of blood volume and in double-labeling procedure for studying axonal branching. It acts as an inhibitor of L-glutamate and kainate receptor-mediated currents. It is also a P2X-selective purinoceptor antagonist. It is also useful in molecular biology.


ChEBI: An organic sodium salt that is the tetrasodium salt of 6,6'-{(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis[diazene-2,1-diyl]}bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate). It is sometimes used as a counterstain, especially in fluorescent methods to suppr ss background autofluorescence.


3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-1,3-disulfonic acid (2 Moore) coupled.

General Description

Blue crystals with a greenish-bronze luster or a black powder.

Air & Water Reactions

Azo dyes can be explosive when suspended in air at specific concentrations. Soluble in water.

Reactivity Profile

Direct Blue 53 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 53 can react with strong reducing and oxidizing agents.

Fire Hazard

Flash point data for Direct Blue 53 are unavailable; however, Direct Blue 53 is probably combustible.

Biological Activity

Potent inhibitor of L-glutamate uptake into synaptic vesicles. Also inhibits AMPA and kainate receptor-mediated currents (IC 50 values are 220 and 150 nM respectively). P2X-selective purinoceptor antagonist.

Safety Profile

Poison by intraperitoneal route.Moderately toxic by intravenous route. An experimentalteratogen. Other experimental reproductive effects.Questionable carcinogen with experimental tumorigenicdata. Mutation data reported. When heated todecomposition it

in vitro

evans blue inhibits the kainate-mediated responses of the non-nmda receptor subunits (giurl, g1ur1,2, g1url,3, and g1ur2,3) expressed in xenopus oocytes without the response of glur3 and g1ur6 at low concentrations (ic50= 355 nm for the subunit combination glur1,2). this pocess was partially reversible without competing with kainate for the agonist binding site [1]. in whole-cell patch clamp recordings of transfected human embryonic kidney 293 cells, evans blue is a potent inhibitor of glutamate-evoked currents mediated by the kainate-type receptor giur6 as long as the ampa-type receptor glurl. interestingly, pretreating with the lectin concanavalin cells recorded relatively little eb inhibition of giur6 currents, eliminating kainate receptor desensitization. in addition to decreasing glur6-mediated peak current amplitude, eb significantly changed receptor desensitization by slowing the rate of onset by ~2-fold (1 m eb) and the rate of recovery by ~2-fold (0.1 p.m eb), and enhancing the steady state to peak current amplitude ratio by ~50-fold (1 m eb) [2].

IC 50

220 and 150 nm for glurl and giur6, respectively.


Room temperature

Properties and Applications

dark green light blue. Soluble in water for bright blue. The strong sulfuric acid for blue-ray green, diluted for light yellow. The dye solution to join hydrochloric acid as bright blue; Add sodium hydroxide solution for the purple and red.


Acid Resistance

Alkali Resistance

Light Fastness
















[1] roseth s, fykse em, fonnum f. uptake of l-glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. j neurochem. 1995 jul;65(1):96-103.
[2] price cj, raymond la. evans blue antagonizes both alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate and kainate receptors and modulates receptor desensitization. mol pharmacol. 1996 dec; 50 (6):1665-71.



1:Can I get some samples before bulk order?
Most products provide free samples, but the shipping cost be paid by customers.


2: What's your MOQ?
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3: Which kind of payment terms do you accept?
PI will be sent first after confirmation of order,enclosed our bank information.Payment by T/T, Western Union,D/P, ETC.

4.How to place an order?
You can contact me through Trademanager, WhatsApp, Email and other contact methods, tell me the product and quantity you need, and then we will give you a quote.

5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.

6:How do you treat quality complaint?
A:First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.


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