|
Product Details:
|
| Product Name: | Alcian Blue 8GX | CAS NO: | 33864-99-2 |
|---|---|---|---|
| Water Solubility: | Moderately Soluble | Form: | Powder |
| Package: | 10g,50g,100g,1kg | Formula: | C56H68CuN16S4.4Cl |
| Color: | Purple | Composition: | Dye Content, 45-65% |
| High Light: | Alcian Blue Biochemical Reagents,CAS 33864-99-2 Biochemical Reagents |
||
CAS 33864-99-2 Alcian Blue 8GX powder
|
Product Name: |
Alcian Blue 8GX |
|
CAS: |
33864-99-2 |
|
MF: |
C56H68CuN16S4.4Cl |
|
MW: |
1298.86 |
|
EINECS: |
251-705-7 |
|
Product Categories: |
marker;Copper phthalocyanine;Dyes and Pigments;Organometallics |
|
Mol File: |
33864-99-2.mol |
|
|
|
|
Melting point |
148 °C |
|
storage temp. |
room temp |
|
solubility |
H2O: soluble1mg/mL |
|
Colour Index |
74240 |
|
form |
powder |
|
color |
Purple |
|
Water Solubility |
moderately soluble |
|
λmax |
615 nm |
|
Biological Applications |
Glycoconjugates assay; hyaluronic acid assay; hybridization assay; cell agglutionation; detecting humic acid,detecting pancreatic b-granules; locomotion of amoeba,quantitative determination of glycosaminoglycans |
|
Chemical Properties |
dark blue powder |
|
Uses |
Alcian blue 8GX is primarily used for demonstrating acid mucopolysaccharides, which it does quite selectively. Alcian Blue 8GX is also suitable for detection of glycoproteins on nitrocellulose and in PAGE gels. |
|
Uses |
Alcian Blue 8GX is widely utilized as a heteroglycan stain for acidic, neutral, sulfated and phosphate polysaccharides and glycosaminoglycans in tissues. It finds application in both light and electron microscopy. It serves as an adhesive to stick glycol methacrylate sections to glass slides. Due to its large aromatic surface, it is used as a coating agent and modifies the property on the surfaces. It acts as a gelling agent for lubricating fluids and is also used in the modification of carbon paste electrode which promotes redox behavior and stability of electrode. |
|
Uses |
Alcian Blue 8GX is used as a heteroglycan stain for neutral, sulfated and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices. |
|
Biochem/physiol Actions |
Alcian Blue 8GX is used as a heteroglycan stain for neutral, sulfated and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices. |
General description
Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.
Application
Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.
It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models.
It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species.
Features and Benefits
Desired staining patterns can be obtained by manipulating pH and salt concentrations.
Principle
The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.
Contact Person: Maggie Ma
Tel: +0086 188 7414 9531
