CAS 302-95-4 Sodium Deoxycholate Supply Industrial Fine Chemicals

Sodium deoxycholate is a bile salt that is commonly used in biological and biochemical research. It is derived from cholic acid and is a key component of the bile produced by the liver. Sodium deoxycholate has several applications and is primarily used as a reagent in protein extraction, solubilization, and purification processes. Here are some important points about sodium deoxycholate:

1. Solubilization of proteins: Sodium deoxycholate is a powerful detergent that can solubilize and disrupt the lipid bilayer of cell membranes. It is often used in protein extraction protocols to lyse cells and release cellular proteins. Sodium deoxycholate can effectively solubilize membrane-associated proteins, enabling their subsequent isolation and analysis.

2. Protein purification: Sodium deoxycholate is used in various protein purification techniques, such as chromatography and affinity purification. It can be employed to disrupt protein-protein interactions or to remove unwanted contaminants from protein samples.

3. Detergent properties: Sodium deoxycholate is an ionic detergent with both hydrophilic and hydrophobic regions. Its structure allows it to interact with hydrophobic regions of proteins, disrupting noncovalent interactions and aiding in protein solubilization. It can also help maintain the solubility of proteins in aqueous solutions.

4. Membrane permeabilization: Sodium deoxycholate can permeabilize cell membranes, allowing the entry of certain molecules or ions into cells. This property is utilized in various applications, including in vitro studies of cellular processes and drug delivery systems.

5. Compatibility: Sodium deoxycholate is compatible with many common laboratory techniques and reagents used in protein analysis, such as gel electrophoresis and enzyme assays. However, its use may be limited in some cases, as it can interfere with certain assays or enzymatic activities.

6. Cautions: Sodium deoxycholate can be irritating to the skin, eyes, and respiratory system. It should be handled with appropriate care, including the use of protective gloves and eyewear. Additionally, it is important to follow proper waste disposal procedures when working with sodium deoxycholate.

Overall, sodium deoxycholate is a versatile reagent in protein research, widely used for protein extraction, solubilization, and purification purposes. Its detergent properties and ability to disrupt lipid membranes make it a valuable tool in various experimental techniques.