Product Details:
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Name: | TMB 3,3′,5,5′-Tetramethylbenzidine | CAS: | 54827-17-7 |
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Appearance: | Powder | Quality: | Refined |
Solubility : | Slightly Soluble. <0.1 G/100 ML At 20 ºC | ||
High Light: | 54827-17-7 In Vitro Diagnostic Reagents,3 3′ 5 5′-Tetramethylbenzidine,TMB In Vitro Diagnostic Reagents |
Basic information |
Product Name: | TMB(3,3′,5,5′-Tetramethylbenzidine) |
CAS: | 54827-17-7 |
MF: | C16H20N2 |
MW: | 240.34 |
EINECS: | 259-364-6 |
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Tetramethylbenzidine Chemical Properties |
Melting point | 168-171 °C(lit.) |
Boiling point | 100 °C |
density | 1 |
refractive index | 1.5519 (estimate) |
storage temp. | 2-8°C |
solubility | Slightly soluble. <0.1 g/100 mL at 20°C. |
pka | 4.49±0.10(Predicted) |
Water Solubility | Slightly soluble. <0.1 g/100 mL at 20 ºC |
Sensitive | Light Sensitive |
BRN | 2808541 |
Stability: | Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents. |
Usage And Synthesi |
Description |
This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product. |
Application |
3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase: to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectant; to measure the levels of α-hemolysin toxin; in imaging bound IgGs; |
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