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Product Details:
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| Name: | 5,5′-Dithiobis(2-nitrobenzoic Acid) DTNB | CAS: | 69-78-3 |
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| Form: | Powder | Storage Conditions : | Keep In Dark Place,Sealed In Dry,Room Temperature |
| Suitability: | Suitable For Determination Of Sulfhydryl Groups | ||
| High Light: | 69-78-3 In Vitro Diagnostic Reagents,DTNB In Vitro Diagnostic Reagents,5 5′-Dithiobis(2-Nitrobenzoic Acid) |
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DTNB CAS 69-78-3 In Vitro Diagnostic Reagents 5,5′-Dithiobis(2-Nitrobenzoic Acid)
| Basic information |
| Product Name: | 5,5′-Dithiobis(2-nitrobenzoic acid) |
| CAS: | 69-78-3 |
| MF: | C14H8N2O8S2 |
| MW: | 396.35 |
| EINECS: | 200-714-4 |
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| 5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties |
| Melting point | 240-245 °C (dec.)(lit.) |
| Boiling point | 671.9±55.0 °C(Predicted) |
| density | 1.67 |
| refractive index | 1.6900 (estimate) |
| storage temp. | Keep in dark place,Sealed in dry,Room Temperature |
| solubility | Do you have solubility information on this product that you would like to share |
| pka | 1.57±0.25(Predicted) |
| form | powder |
| Water Solubility | Soluble in water and ethanol. |
| BRN | 1896821 |
| Stability: | Stable. Incompatible with strong bases, strong oxidizing agents. |
| InChIKey | KIUMMUBSPKGMOY-UHFFFAOYSA-N |
| CAS DataBase Reference | 69-78-3(CAS DataBase Reference) |
| Usage And Synthe |
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Description |
5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3. |
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Application |
Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues. It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. |
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