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Product Details:
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| Name: | Guanidine Hydrochloride | CAS: | 50-01-1 |
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| Form: | Crystalline Powder | Storage Conditions : | Room Temp |
| Color: | White | Impurities: | ≤0.3% Water (Karl Fischer) |
| High Light: | 50-01-1 In Vitro Diagnostic Reagents,Guanidine Hydrochloride HCL White Color,50-01-1 Guanidine Hydrochloride |
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CAS 50-01-1 In Vitro Diagnostic Reagents Guanidine Hydrochloride HCL White Color
| Guanidine hydrochloride HCLBasic information |
| Product Name: | Guanidine hydrochloride |
| Synonym(s): | Aminoformamidine hydrochloride, Guanidinium chloride |
| CAS: | 50-01-1 |
| MF: | CH5N3.ClH |
| MW: | 95.53 |
| EINECS: | 200-002-3 |
| HS Code: | 29252000 |
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| Guanidine hydrochloride Chemical Properties |
| Melting point | 180-185 °C(lit.) |
| density | 1.18 g/mL at 25 °C(lit.) |
| refractive index | n |
| storage temp. | room temp |
| solubility | H2O: 6 M, clear, colorless |
| color | White |
| Odor | Odorless |
| PH | 4.5-5.5 (100g/l, H2O, 20℃) |
| PH Range | 4.5 - 6.0 at 573 g/l at 25 °C |
| Water Solubility | 2280 g/L (20 ºC) |
| λmax | λ: 260 nm Amax: 0.040 λ: 280 nm Amax: 0.025 |
| Sensitive | Hygroscopic |
| Merck | 14,4562 |
| BRN | 3591990 |
| Stability: | Stable. Hygroscopic. Incompatible with strong oxidizing agents. |
| InChIKey | PJJJBBJSCAKJQF-UHFFFAOYSA-N |
| CAS DataBase Reference | 50-01-1(CAS DataBase Reference) |
| EPA Substance Registry System | Guanidine monohydrochloride (50-01-1) |
| Guanidine hydrochloride Usage And Synthesis |
| Application: | Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. |
| Uses: | in extraction during protein fractionation of ATDC5 cell lines; incubate the device chip to release prostate-specific antigen and for regeneration of aptamer; as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study; dissociate nucleoproteins and inhibit RNase in RNA isolation |
| Uses: | as a component of the extraction buffer for the extraction of proteoglycans; as a chemical additive to study its effective absorbance spectra in structural analysis; |
| Caution: | May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. |
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Preparation Note: |
In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes. The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M. |
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