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Cas 53-79-2 Puromycin Powder ISO Certificated

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Cas 53-79-2 Puromycin Powder ISO Certificated

Cas 53-79-2 Puromycin Powder ISO Certificated
Cas 53-79-2 Puromycin Powder ISO Certificated Cas 53-79-2 Puromycin Powder ISO Certificated Cas 53-79-2 Puromycin Powder ISO Certificated Cas 53-79-2 Puromycin Powder ISO Certificated Cas 53-79-2 Puromycin Powder ISO Certificated

Large Image :  Cas 53-79-2 Puromycin Powder ISO Certificated

Product Details:
Place of Origin: China
Brand Name: yunbangpharm
Certification: ISO9001/SGS
Model Number: YB077
Payment & Shipping Terms:
Minimum Order Quantity: To Be Negotiated
Price: To Be Negotiated
Packaging Details: 1kg 5kg 25kg
Delivery Time: 3-7Working Days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram

Cas 53-79-2 Puromycin Powder ISO Certificated

Description
Product Name: Puromycin CAS: 53-79-2
Form: Powder Certificate: ISO
Samples: Available
High Light:

Cas 53-79-2 Puromycin

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ISO Puromycin Powder

 

Puromycin Stylomycin Cas 53-79-2

 

CAS: 53-79-2
MF: C22H29N7O5
MW: 471.51

 

Melting point 175.5-177°
alpha D25 -11° (ethanol)
Boiling point 572.65°C (rough estimate)
density 1.3119 (rough estimate)
refractive index 1.7010 (estimate)
RTECS AU7350000
pka 6.8, 7.2(at 25℃)
form Liquid
Water Solubility Soluble in water (50 mg/ml at 20°C).
Description Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It is a very common antibiotic routinely used by scientists in biomedical research to select cells modified by genetic engineering. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. The antibiotic inhibits the growth of Gram-positive bacteria and various animal and insect cells. Fungi and Gram-negative bacteria are resistant due to the low permeability of the antibiotic. For more than 30 years, puromycin has been widely used as a basic tool for studying protein synthesis. Now, puromycin hydrochloride is particularly useful for the selection of cell types harboring plasmids carrying puromycin resistance genes. Puromycinresistant cells express pac gene, which encodes an N-acetyl puromycin transferase. The pac gene can be mobilized on a plasmid and used to transfect a host cell in an attempt to provide resistance; therefore, puromycin can be used in gene selections for mammalian host cells.
This aminonucleoside antibiotic causes premature chain termination during translation in the ribosomes. Part of the molecule resembles the 30 end of the aminoacylated tRNA. It enters the A site and transfers to the growing chain, causing premature chain release. The exact mechanism of action is unknown, but the 30 position contains an amide linkage instead of the normal ester linkage of tRNA; the amide bond makes the molecule much more resistant to hydrolysis and thus causes the ribosome to become stopped. It is not selective for either prokaryotes or eukaryotes.
Also of note, puromycin is critical in mRNA display as it allows the growing peptide chain to be covalently bonded to its own mRNA template. Additionally, puromycin is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase (metallopeptidase). The mechanism of inhibition is not well understood; however, puromycin can be used to distinguish between aminopeptidase M (active) and cytosol alanyl aminopeptidase (inhibited by puromycin) and therefore extremely useful in biochemistry and nephrology research.

 

 

Cas 53-79-2 Puromycin Powder ISO Certificated 0 

 

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Contact Person: Maggie Ma

Tel: +0086 188 7414 9531

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