Proteinase K CAS 39450-01-6 Reagents Enzymes SGS Approved Biochemical

Name: Proteinase K CAS 39450-01-6 Reagents Enzymes SGS Approved Biochemical
Brand: Hunan Yunbang Biotech Inc.
SKU: YB117
Availability: InStock

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Proteinase K CAS 39450-01-6 Reagents Enzymes SGS Approved Biochemical

Large Image : Proteinase K CAS 39450-01-6 Reagents Enzymes SGS Approved Biochemical

Product Details:

Place of Origin:	China
Brand Name:	YunbangPharm
Certification:	ISO9001/SGS
Model Number:	YB117

Payment & Shipping Terms:

Minimum Order Quantity:	To Be Negotiated
Price:	To Be Negotiated
Packaging Details:	1g 10g 100g
Delivery Time:	3-7 Working Days
Payment Terms:	L/C, D/A, D/P, T/T, Western Union

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Proteinase K CAS 39450-01-6 Reagents Enzymes SGS Approved Biochemical

Description

Name:	Proteinase K	CAS:	39450-01-6
Form:	Powder	Foreign Activity:	DNAse, Nickase And RNAse, None Detected
Storage Temp.:	−20°C
Highlight:	Biochemical Proteinase K , Enzymes Proteinase K , CAS 39450-01-6

Proteinase K 39450-01-6 Biochemical Reagent Enzymes

Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Analysis Note

Appearance (color): white
Appearance (description): lyophilisate
Activity (hemoglobin; pH 7.5; 37 °C): ≥ 30.0 mAnsonU/mg
Spec. activity (calc. on protein): ≥ 40 mAnsonU/mg
DNases (Nicking activity; pBR 322; 6 h; 37 °C): not detectable
RNases (RNA; 2 h; 37°C): not detectable

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Proteinase K CAS 39450-01-6 Reagents Enzymes SGS Approved Biochemical

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Proteinase K CAS 39450-01-6 Reagents Enzymes SGS Approved Biochemical

Proteinase K CAS 39450-01-6 Reagents Enzymes SGS Approved Biochemical

Biochemical Proteinase K

Enzymes Proteinase K

CAS 39450-01-6

Application

Biochem/physiol Actions

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