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Product Details:
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Product Name: | Alizarin-3-methyliminodiacetic Acid | Cas No: | 3952-78-1 |
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Molecular Formula: | C19H15NO8 | EINECS: | 223-544-2 |
Colour: | Brown | Boiling Point: | ~185 °C (dec.) |
Melting Point: | 511.79°C (rough Estimate) | Form: | Powder |
Highlight: | Alizarin-3-Methyliminodiacetic Acid,CAS 3952-78-1 Biological Buffers |
CAS 3952-78-1 Alizarin-3-methyliminodiacetic acid
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Product Name: | ALIZARIN COMPLEXONE |
Synonyms: | (((3,4-dihydroxy-2-anthraquinonyl)methyl)imino)di-aceticaci;[(3,4-Dihydroxy-2-anthraquinonyl)methyl]iminod;1,2-dihydroxy-anthrachinon-3-methylen-iminodiessigsaeure;Alizarin Fluorine Blue test solution(ChP);2-[carboxymethyl-[(3,4-dihydroxy-9,10-dioxo-2-anthracenyl)methyl]amino]acetic acid;ALIZARIN COMPLEXONE;Aizarin-3-N,N-diaceticacid;Alizarincompiexone |
CAS: | 3952-78-1 |
MF: | C19H15NO8 |
MW: | 385.32 |
EINECS: | 223-544-2 |
Product Categories: | Photometric Reagents (Complexone);Analytical Chemistry;Anthraquinones;Chelating Reagents;Complexones;Hydroxyanthraquinones |
Mol File: | 3952-78-1.mol |
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Melting point | ~185 °C (dec.) |
Boiling point | 511.79°C (rough estimate) |
density | 1.4951 (rough estimate) |
refractive index | 1.5000 (estimate) |
storage temp. | 2-8°C |
solubility | DMF: 10 mg/ml; DMSO: 10 mg/ml; DMSO:PBS (pH 7.2) (1:7): 0.125 mg/ml |
pka | 1.71±0.10(Predicted) |
form | Fine Powder |
color | Brown |
Water Solubility | Slightly soluble (0.2 g/L) |
BRN | 2190028 |
Stability: | Incompatible with strong oxidizing agents. |
InChI | InChI=1S/C19H15NO8/c21-13(22)7-20(8-14(23)24)6-9-5-12-15(19(28)16(9)25)18(27)11-4-2-1-3-10(11)17(12)26/h1-5,25,28H,6-8H2,(H,21,22)(H,23,24) |
InChIKey | PWIGYBONXWGOQE-UHFFFAOYSA-N |
SMILES | C(O)(=O)CN(CC(O)=O)CC1=C(O)C(O)=C2C(=C1)C(=O)C1=C(C=CC=C1)C2=O |
CAS DataBase Reference | 3952-78-1(CAS DataBase Reference) |
EPA Substance Registry System | Glycine, N-(carboxymethyl)-N-[(9,10-dihydro-3,4-dihydroxy-9,10-dioxo-2-anthracenyl)methyl]- (3952-78-1) |
Description | Alizarin-3-methyliminodiacetic acid is a colorimetric dye for the detection of fluoride ions. It reacts with fluoride to form a lilac-blue complex which can be quantified colorimetrically at 620 nm to determine fluoride concentration. Alizarin-3-methyliminodiacetic acid has been used to visualize fluoride deposition and bone mineralization during development in medaka larvae. It is also an inhibitor of inducible nitric oxide synthase (iNOS; IC50 = 35 nM). |
Chemical Properties | Brown-orange crystals, insoluble in water |
Uses | Alizarin is the main ingredient for the manufacture of the madder lake pigments known to painters as Rose madder and Alizarin crimson. A notable use of alizarin in modern times is as a staining agent in biological research because it stains free calcium and certain calcium compounds a red or light purple color. Alizarin Red is used in a biochemical assay to determine, quantitatively by colorimetry, the presence of calcific deposition by cells of an osteogenic lineage. |
Uses | Alizarin Complexone is used for the determination of fluorine and other anions. ATA is also a potent inhibitor of protein-nucleic acid interactions. |
Uses | Alizarin-3-methyliminodiacetic acid has been used:
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Definition | ChEBI: A dihydroxyanthraquinone compound in which the hydroxy groups are at C-1 and C-2 and which has a bis[(carboxymethyl)amino]methyl substituent at the 3-position. |
General Description | Alizarin-3-methyliminodiacetic acid is used in the colorimetric detection of fluorine. It is a dye employed to stain mineralized bones of the preserved specimen from vertebrates. |
Hazard | Anthraquinones are toxic by ingestion: vomiting, diarrhea, kidney and liver damage, and coma; Deaths have been reported after children ingested Rhamnus berries; Has antiretroviral activity; An irritant; Harmful by ingestion, inhalation, or skin absorption; Toxic if swallowed. |
Purification Methods | It is purified by suspending it in 0.1M NaOH (1g in 50mL), filtering the solution and extracting alizarin with 5 successive portions of CH2Cl2. Then add HCl dropwise to precipitate the reagent, stirring the solution in an ice bath. Filter the precipitate onto a glass filter, wash it with cold water and dry it in a vacuum desiccator over KOH [Ingman Talanta 20 135 1973, Beilstein 14 IV 931]. |
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