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Product Details:
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| Product Name: | Hematoxylin | CAS NO: | 517-28-2 |
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| Appearance: | Powder | Sample: | Available |
| Formula: | C16H14O6 | Solubility: | Soluble In 95% Ethanol(1 Mg/mL). |
| EINECS NO: | 208-237-3 | Colour: | Yellow To Brown |
CAS NO 517-28-2 Hematoxylin powder
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Product Name: |
Hematoxylin |
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Synonyms: |
GILL 2 METHOD HEMATOXYLIN STAIN;GILL HEMATOXYLIN SOLUTION NO II;GILL'S HEMATOXYLIN NO 2;GILL'S HEMATOXYLIN SOLUTION NO 2;GILLS II HAEMATOXYLIN;HEMATOXYLIN STAIN, GILL 2;HEMATOXYLIN SOLUTION GILL NO 2;HEMATOXYLIN, GILL II |
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CAS: |
517-28-2 |
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MF: |
C16H14O6 |
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MW: |
302.28 |
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EINECS: |
208-237-3 |
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Product Categories: |
Polymethine;Flavone;Cytology StainsHematology and Histology;marker;Inhibitors;Miscellaneous;Colours, Dyes, Indicators & Pigments;Hematology and Histology;Routine Histology Stains |
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Mol File: |
517-28-2.mol |
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Melting point |
200 °C (dec.)(lit.) |
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Boiling point |
363.32°C (rough estimate) |
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density |
1.2514 (rough estimate) |
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vapor pressure |
0-0Pa at 20-25℃ |
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refractive index |
1.4600 (estimate) |
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storage temp. |
Keep in dark place,Inert atmosphere,Room temperature |
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solubility |
Soluble in 95% ethanol(1 mg/mL). |
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Colour Index |
75290 |
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pka |
6.7(at 25℃) |
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form |
Powder/Solid |
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color |
Yellow to Brown |
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PH Range |
Red (0.0) to yellow (1.0);Pale yellow (5.0) to violet (6.0) |
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Water Solubility |
SOLUBLE IN HOT WATER |
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Sensitive |
Light Sensitive |
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λmax |
292nm, 445nm, 560nm |
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Merck |
14,4637 |
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BRN |
91399 |
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Stability: |
Stable, but may discolour on exposure to light. Incompatible with strong oxidizing agents. |
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Major Application |
Plasma displays, textiles, hair dyes, identifying fresh and stale rice, diag-nosing cancer progression, detecting nosing cancer progression, cervical disease, central nervous system malfunctions, detecting genes, breast cancer, collagen in a tissue sample, apoptosis, demyelinating diseases, antigens, treatment of age-related macular degeneration, burns, prostate cancer, diabetesand obesity, viral diseases, neoplasms, peripheral neural and vascular ailments, skin disorders, biotechnological applications, reference standard materials for cytology, histology andimmunohistochemistry |
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InChIKey |
WZUVPPKBWHMQCE-UHFFFAOYSA-N |
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LogP |
0.3 at 30℃ and pH6.9 |
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CAS DataBase Reference |
517-28-2 |
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EPA Substance Registry System |
Hematoxylin (517-28-2) |
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Chemical Properties |
light brown crystals or powder |
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Uses |
Colorant in inks, biological stains. |
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Uses |
Hematoxylin and eosin together make up Hematoxylin and eosin stain, one of the most commonly used stains in histology. Metal-haematein complexes are used to stain cell nuclei prior to examination under a microscope. Structures that stain with iron- or aluminum-haematein are often called basophilic, even though the mechanism of the staining is different from that of staining with basic dyes. |
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Definition |
ChEBI: (+)-haematoxylin is a haematoxylin. It is an enantiomer of a (-)-haematoxylin. |
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General Description |
White to yellowish crystals that redden on exposure to light. |
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Air & Water Reactions |
Soluble in hot water, slightly water soluble in cold . |
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Reactivity Profile |
Sensitive to light. . |
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Hazard |
May be carcinogenic. |
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storage |
Store at RT |
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Purification Methods |
Hematoxylin recrystallises from H2O (as trihydrate) in white-yellow crystals which become red on exposure to light and then melt at 100-120o. It has been recrystallised from Me2CO/*C6H6. It has been recrystallised as well from dilute aqueous NaHSO3 until colourless and is soluble in alkali, borax and glycerol. Store it in the dark below 0o. [Morsingh & Robinson Tetrahedron 26 182 1970, Dann & Hofmann Chem Ber 98 1498 1955, Beilstein 17/8 V 469.] |
General description
Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.
Application
Hematoxylin has been used:
to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples
as a nuclear counterstain to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent
to stain lung tissue for histological analysis
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Advantage
1. 90days quality guarantee for exchange.
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