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Product Details:
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| Product Name: | Evans Blue | CAS NO: | 314-13-6 |
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| Form: | Powder | Sample: | Available |
| Formula: | C34H29N6NaO14S4 | Colour: | Dark Brown |
| Water Solubility: | 280 G/L (20 ºC) | Solubility: | DMSO (Slightly), Water (Slightly) |
| High Light: | Direct Blue 314-13-6,Direct Blue Biochemical Reagents,280g/L Water Solubility Direct Blue |
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Evans Blue CAS NO 314-13-6 Dye Content ≥75 % Biochemical Reagents
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Product Name: |
Evans Blue |
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CAS: |
314-13-6 |
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MF: |
C34H29N6NaO14S4 |
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MW: |
896.86 |
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EINECS: |
206-242-5 |
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Product Categories: |
MATULANE;Biological Dye;Organics;Glutamate receptor |
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Mol File: |
314-13-6.mol |
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| Chemical Properties | brownish crystalline powder | ||||||||||||||||||||
| Uses | antineoplastic | ||||||||||||||||||||
| Uses | Evans Blue is used for estimation of blood volume and in double-labeling procedure for studying axonal branching. It acts as an inhibitor of L-glutamate and kainate receptor-mediated currents. It is also a P2X-selective purinoceptor antagonist. It is also useful in molecular biology. | ||||||||||||||||||||
| Definition | ChEBI: An organic sodium salt that is the tetrasodium salt of 6,6'-{(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis[diazene-2,1-diyl]}bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate). It is sometimes used as a counterstain, especially in fluorescent methods to suppr ss background autofluorescence. | ||||||||||||||||||||
| Preparation | 3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-1,3-disulfonic acid (2 Moore) coupled. | ||||||||||||||||||||
| General Description | Blue crystals with a greenish-bronze luster or a black powder. | ||||||||||||||||||||
| Air & Water Reactions | Azo dyes can be explosive when suspended in air at specific concentrations. Soluble in water. | ||||||||||||||||||||
| Reactivity Profile | Direct Blue 53 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 53 can react with strong reducing and oxidizing agents. | ||||||||||||||||||||
| Fire Hazard | Flash point data for Direct Blue 53 are unavailable; however, Direct Blue 53 is probably combustible. | ||||||||||||||||||||
| Biological Activity | Potent inhibitor of L-glutamate uptake into synaptic vesicles. Also inhibits AMPA and kainate receptor-mediated currents (IC 50 values are 220 and 150 nM respectively). P2X-selective purinoceptor antagonist. | ||||||||||||||||||||
| Safety Profile | Poison by intraperitoneal route.Moderately toxic by intravenous route. An experimentalteratogen. Other experimental reproductive effects.Questionable carcinogen with experimental tumorigenicdata. Mutation data reported. When heated todecomposition it | ||||||||||||||||||||
| in vitro | evans blue inhibits the kainate-mediated responses of the non-nmda receptor subunits (giurl, g1ur1,2, g1url,3, and g1ur2,3) expressed in xenopus oocytes without the response of glur3 and g1ur6 at low concentrations (ic50= 355 nm for the subunit combination glur1,2). this pocess was partially reversible without competing with kainate for the agonist binding site [1]. in whole-cell patch clamp recordings of transfected human embryonic kidney 293 cells, evans blue is a potent inhibitor of glutamate-evoked currents mediated by the kainate-type receptor giur6 as long as the ampa-type receptor glurl. interestingly, pretreating with the lectin concanavalin cells recorded relatively little eb inhibition of giur6 currents, eliminating kainate receptor desensitization. in addition to decreasing glur6-mediated peak current amplitude, eb significantly changed receptor desensitization by slowing the rate of onset by ~2-fold (1 m eb) and the rate of recovery by ~2-fold (0.1 p.m eb), and enhancing the steady state to peak current amplitude ratio by ~50-fold (1 m eb) [2]. | ||||||||||||||||||||
| IC 50 | 220 and 150 nm for glurl and giur6, respectively. | ||||||||||||||||||||
| storage | Room temperature | ||||||||||||||||||||
| Properties and Applications | dark green light blue. Soluble in water for bright blue. The strong sulfuric acid for blue-ray green, diluted for light yellow. The dye solution to join hydrochloric acid as bright blue; Add sodium hydroxide solution for the purple and red.
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| references | [1] roseth s, fykse em, fonnum f. uptake of l-glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. j neurochem. 1995 jul;65(1):96-103. [2] price cj, raymond la. evans blue antagonizes both alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate and kainate receptors and modulates receptor desensitization. mol pharmacol. 1996 dec; 50 (6):1665-71. |
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General description
Evans blue (EB) C.I. 23860 is a synthetic bis-azo dye also known as T-1824 and Direct Blue 53. EB dye with higher water solubility has a strong affinity towards serum albumin, making it a high molecular weight protein tracer.
Application
Evans blue is a well-known vital stain with applications in histology and fluorescent microscopy. Evans blue has been used to check cell viability and study blood-brain barrier (BBB) permeability. Additional applications include the demonstration of specific pituitary cell types, selective staining of neutrophil plasma membranes, and fluorescence microscopy as a quenching agent to suppress unwanted autofluorescence.
Biochem/physiol Actions
Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.
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