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Product Details:
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| Product Name: | Acridine Orange Base | CAS NO: | 494-38-2 |
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| Form: | Powder | Sample: | Available |
| Formula: | C17H19N3 | Colour: | Yellow Needles From EtOH |
| Storage Condition: | Sealed In Dry,Room Temperature | Appearance: | Light Sensitive |
| Highlight: | 494-38-2 Biochemical Reagents,Acridine Orange Base 494-38-2,Acridine Orange Base Powder |
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Acridine Orange Base CAS NO 494-38-2 Biochemical Reagents
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Product Name: |
ACRIDINE ORANGE |
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Synonyms: |
N3,N3,N6,N6-TetraMethylacridine-3,6-diaMine;3,6-Bis(dimethylamino)acridine N,N,N',N'-Tetramethyl-3,6-acridinediamine;Acridine Orange Stain;brilliantacridineorangee;compound with zinc-chloride and hydrogen chloride;N3,N3,N6,N6-tetramethyl-acridine-3,6-diyldiamine;3,6-bis-(dimethylamino)akridin;3,6-di(dimethylamino)acridine |
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CAS: |
494-38-2 |
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MF: |
C17H19N3 |
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MW: |
265.35 |
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EINECS: |
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Product Categories: |
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Mol File: |
494-38-2.mol |
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Melting point |
165 °C (dec.)(lit.) |
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Boiling point |
469℃ |
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density |
1.169 |
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refractive index |
1.6080 (estimate) |
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Fp |
237℃ |
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storage temp. |
Sealed in dry,Room Temperature |
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solubility |
DMF: 2 mg/ml; DMSO: 20 mg/ml; Ethanol: 0.3 mg/ml; PBS (pH 7.2): 1 mg/ml |
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Colour Index |
46005 |
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pka |
9.97±0.30(Predicted) |
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form |
A crystalline solid |
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color |
Yellow needles from EtOH |
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Stability: |
Light Sensitive |
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IARC |
3 (Vol. 16, Sup 7) 1987 |
General description
Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye.
Application
Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.
Purification Methods
The double salt with ZnCl2 (6g) is dissolved in water (200mL) and stirred with four successive portions (12g each) of Dowex-50 ion-exchange resin (K+ form) to remove the zinc. The solution is then concentrated in vacuum to 20mL, and 100mL of ethanol is added to precipitate KCl which is removed. Ether (160mL) is added to the solution from which, on chilling, the dye crystallises as its chloride. It is separated by centrifugation, washed with chilled ethanol and ether, and dried under vacuum, before being recrystallised from ethanol (100mL) by adding ether (50mL), and chilling. Yield 1g. [Pal & Schubert J Am Chem Soc 84 4384 1962]. It was recrystallised twice as the free base from ethanol or methanol/water by dropwise addition of NaOH (less than 0.1M). The precipitate was washed with water and dried under vacuum. It was dissolved in CHCl3 and chromatographed on alumina: the main sharp band was collected, concentrated and cooled to -20o. The precipitate was filtered off, dried in air, then dried for 2hours under vacuum at 70o. [Stone & Bradley J Am Chem Soc 83 3627 1961, Blauer & Linschitz J Phys Chem 66 453 1962, Albert J Chem Soc 244 1947, Beilstein 22 III/IV 5490, 22/11 V 326.]
FAQ
1:Can I get some samples before bulk order?
Most products provide free samples, but the shipping cost be paid by customers.
2: What's your MOQ?
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3: Which kind of payment terms do you accept?
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5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.
6:How do you treat quality complaint?
A:First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.
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Tel: +0086 188 7414 9531
