CAS 6850-28-8 Tris Acetate Buffer THAM Acetate Tris(Hydroxymethyl)Aminomethane Acetate Salt 99%

Tris(hydroxymethyl)aminomethane acetate salt, also known as Tris acetate or THAM acetate, is a chemical compound commonly used as a buffering agent in biochemical and molecular biology applications. It is a salt form of Tris base, where the hydroxyl groups are partially or fully replaced by acetate groups.

Here are some key points about Tris(hydroxymethyl)aminomethane acetate salt:

1. Chemical formula: C4H11NO3 · CH3COOH
2. Molecular weight: Varies depending on the degree of acetate substitution.
3. Buffering capacity: Tris acetate has a pKa value around 8.3, making it effective as a buffering agent in the pH range of approximately 7.0 to 9.0.
4. Buffer preparation: Tris acetate buffer is prepared by dissolving the salt in water and adjusting the pH using a strong acid or base, typically hydrochloric acid (HCl) or sodium hydroxide (NaOH).
5. Applications: Tris acetate buffer is commonly used in various biochemical and molecular biology techniques, including nucleic acid electrophoresis, DNA/RNA extraction, enzyme assays, and protein purification. It provides a stable pH environment to maintain the activity and stability of biological molecules.
6. Gel electrophoresis: Tris acetate buffer is often used as the running buffer in agarose gel electrophoresis for the separation and analysis of nucleic acids. It provides suitable pH conditions for the migration of DNA or RNA fragments through the gel matrix.
7. Enzyme assays: Tris acetate buffer is employed in enzyme assays to maintain the optimal pH for enzymatic reactions. The buffer system helps ensure the accuracy and reliability of enzyme activity measurements.
8. Compatibility: Tris acetate buffer is compatible with many enzymes, proteins, and nucleic acids. However, its use may not be suitable for certain applications where the presence of acetate ions can interfere with specific assays or interactions.

When working with Tris acetate or any chemical compound, it is essential to follow proper safety precautions, including the use of appropriate personal protective equipment (PPE) and adherence to safe handling practices. The specific properties and applications of Tris acetate may vary depending on the concentration, pH, and experimental conditions used.