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Product Details:
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| Name: | 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate Hydrate | Cas No: | 75621-03-3 |
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| Application: | Molecular Biology/Industrial Detergent | Purity: | 99% |
| Classification: | Biological Buffer | Form: | Crystal Powder |
| Color: | White | Samples: | Available |
| Package: | 1kg/5kg/25kg | Transport: | Courier/Air/Sea |
| High Light: | CHAPS Biological Buffers,Biological Buffers Detergent,CAS 75621-03-3 |
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CHAPS Buffer CAS 75621-03-3 Biological Buffers Detergent
| 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate Basic information |
| Product Name: | 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate |
| Synonyms: | CHAPS;CHAPS (3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO)PROPANESULFONATE);3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANE SULPHONATE (ULTRAPURE);3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]PROPANESULFONAT(CHAPS);3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulphonate (Ultrapure) >99.0%;CHAPS, 98+%;CHAPS extrapure;1-Propanaminium, N,N-dimethyl-N-(3-sulfopropyl)-3-[[(3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl]amino]-, inner salt |
| CAS: | 75621-03-3 |
| MF: | C32H58N2O7S |
| MW: | 614.88 |
| EINECS: | 616-246-2 |
| Mol File: | 75621-03-3.mol |
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| 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate Chemical Properties |
| Melting point | 156-158°C (dec.) |
| density | 1.01 g/mL at 20 °C |
| refractive index | 1.6900 (estimate) |
| storage temp. | -20°C |
| solubility | H2O: 50 mg/mL at 20 °C, clear, colorless |
| form | Crystalline Powder |
| color | White |
| Odor | Odorless |
| PH Range | 6 |
| Water Solubility | Soluble in water (10 mg/ml). Soluble in DMF and hot methanol. |
| Sensitive | Hygroscopic |
| 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate Usage And Synthesis |
| Chemical Properties | White crystalline powder |
| Uses | A non-denaturing zwitterionic detergent for membrane biochemistry |
| Uses | Buffer; zwitterionic nondenaturing detergent for solubilizing membrane proteinsCHAPS is used in the laboratory to dissolve biological macromolecules such as proteins. It acts as a non-denaturing solvent used in purification of membrane proteins. It is also useful in conjunction with nonionic detergents like Triton X-100. Further, it is used as a non-denaturing zwitterionic detergent for membrane biochemistry. |
| Uses | Solubilization of membrane proteins. Combines the properties of both the sulfobetaine-type and bile-acid detergents. |
| General Description | CHAPS is a stabilizing agent that can be used to prevent aggregation, precipitation or proteolysis of proteins in solution. |
| Biological Activity | CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. |
| Purification Methods | Triturate this zwitterionic detergent (~50g) with MeOH (200ml), then triturate it with Me2CO (500mL), collect it by vacuum filtration and dry it thoroughly at ~20o. Recrystallise it at 0o from absolute MeOH followed by drying in vacuo to constant weight. It should give one spot with RF 0.32 by TLC on silica gel G in 95% MeOH/5% NH4OH and visualized with iodine vapour; the tertiary amine precursor has RF 0.40. It has low UV absorption (1% in H2O: A280nm is 0.029 and A260nm is 0.035), and the CMC (critical micellar concentration) is 8mM. It is soluble in H2O, MeOH, Me2SO but insoluble in Me2CO, MeCN, hexane, toluene and xylene. [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.] CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate betaine) [82473-24-3] M 630.89, m 184-196o, [ ] D +19o (c 2, H2O), pKEst (1) ~<2 (acidic), pKEst(2) ~9 (basic). Dissolve this zwitterionic detergent (~ 60g) in 40% aqueous MeOH and stir it with the mixed-bed ion-exchange resin RG-501 x 8 (100g, Bio-Rad) until the pH of the supernatant is ~ 7. Filter off the resin and evaporate the filtrate to dryness under reduced pressure. Check for homogeneity by TLC on silica gel G in 95% MeOH/5% NH4OH and visualize the spot with iodine vapour (see CHAPS above). [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.] |
Contact Person: Maggie Ma
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