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Product Details:
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| Name: | N,N-Bis(2-hydroxyethyl)glycine | Cas No: | 150-25-4 |
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| Application: | Bioreagent | Purity: | 99% |
| Classification: | Biological Buffer | Form: | Powder |
| Color: | White | Samples: | Available |
| Package: | 1kg/5kg/25kg | Transport: | Courier/Air/Sea |
| High Light: | Bicine Biological Buffer,Bioreagent Biological Buffer,CAS 150-25-4 |
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Bicine CAS 150-25-4 Bioreagent Biological Buffer Good's buffers
| Bicine Basic information |
| Product Name: | Bicine |
| Synonyms: | n,n-dihydroxyethyl-glycin;Bicine N,N-Bis(2-hydroxyethyl)glycine Diethanolglycine;N,N-DI(2-HYDROXYETHYL)GLYCINE;N,N-BIS(2-HYDROXYETHYL)GLYCINE;N N-DI(HYDROXYETHYL)-B-AMINOACETIC ACID;BICINE[N,N-Di(2-hydroxyethyl)glycine];BICIN (N,N-Bis-(2-hydroxyethyl)-glycin);N,N-Di(2-hydroxyethyl)glycine |
| CAS: | 150-25-4 |
| MF: | C6H13NO4 |
| MW: | 163.17 |
| EINECS: | 205-755-1 |
| Product Categories: | Biochemistry;Good's Buffers;Buffer |
| Mol File: | 150-25-4.mol |
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| Bicine Chemical Properties |
| Melting point | 190 °C (dec.)(lit.) |
| Boiling point | 290.25°C (rough estimate) |
| density | 1.05 g/mL at 20 °C |
| refractive index | 1.4240 (estimate) |
| storage temp. | room temp |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | Powder |
| color | White |
| PH | 4.0-5.5 (25℃, 1M in H2O) |
| PH Range | 7.6 - 9.0 |
| pka | 8.3(at 25℃) |
| Water Solubility | soluble |
| λmax | λ: 260 nm Amax: 0.070 λ: 280 nm Amax: 0.040 |
| Merck | 14,1201 |
| BRN | 1769362 |
| Bicine Usage And Synthesis |
| Chemical Properties | White/clear crystalline powder |
| Uses | To prepare stable substrate solution for the determination of guanine deaminase. The buffer is widely used with the working concentration of 3-100mM. |
| Uses | Biological buffer and chelating agent. |
| Uses | A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described. |
| Purification Methods | Dissolve bicine in a small volume of hot water and precipitate it with EtOH, twice. Repeat once more but treat the aqueous solution with charcoal Purification of Biochemicals — Amino Acids and Peptides and filter before adding EtOH. Also crystallise it from concentrated aqueous solutions. [Torn & Kolthoff J Am Chem Soc 77 2061 1955, Chaberek et al. J Am Chem Soc 75 2185 1953, Beilstein 4 IV 2390.] |
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