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CAS 150-25-4 Bicine Bioreagent Biological Buffer 99% Purity

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CAS 150-25-4 Bicine Bioreagent Biological Buffer 99% Purity

CAS 150-25-4 Bicine Bioreagent Biological Buffer 99% Purity
CAS 150-25-4 Bicine Bioreagent Biological Buffer 99% Purity CAS 150-25-4 Bicine Bioreagent Biological Buffer 99% Purity CAS 150-25-4 Bicine Bioreagent Biological Buffer 99% Purity CAS 150-25-4 Bicine Bioreagent Biological Buffer 99% Purity

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Product Details:
Place of Origin: China
Brand Name: YunbangPharm
Certification: ISO9001
Model Number: YB008
Payment & Shipping Terms:
Minimum Order Quantity: Negotiable
Price: Negotiable
Packaging Details: 1kg/Bottle, 5-25kg/Drum
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T, Western Union
Supply Ability: 200kg

CAS 150-25-4 Bicine Bioreagent Biological Buffer 99% Purity

Description
Name: N,N-Bis(2-hydroxyethyl)glycine Cas No: 150-25-4
Application: Bioreagent Purity: 99%
Classification: Biological Buffer Form: Powder
Color: White Samples: Available
Package: 1kg/5kg/25kg Transport: Courier/Air/Sea
High Light:

Bicine Biological Buffer

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Bioreagent Biological Buffer

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CAS 150-25-4

Bicine, also known as N,N-Bis(2-hydroxyethyl)glycine, is a zwitterionic amino acid commonly used as a buffering agent in biochemical and biological research. It is structurally similar to glycine but contains two hydroxyethyl groups in addition to the amino and carboxyl groups.

Here are some key points about Bicine:

  1. Chemical formula: C6H13NO4
  2. Molecular weight: 163.17 g/mol
  3. Buffering capacity: Bicine has a pKa value of approximately 8.3, making it effective as a buffering agent in the pH range of approximately 7.6 to 9.0.
  4. Solubility: Bicine is highly soluble in water and is also soluble in polar organic solvents like methanol and ethanol.
  5. Applications: Bicine is commonly used in various biochemical and biological applications that require a stable pH environment.
    • Electrophoresis: Bicine is often used as a buffering agent in polyacrylamide gel electrophoresis (PAGE) systems for the separation of proteins and peptides. It helps maintain the appropriate pH for optimal resolution and migration of biomolecules.
    • Enzyme assays: Bicine is employed in enzyme assays to provide a stable pH environment for enzymatic reactions. It helps maintain the activity and stability of enzymes.
    • Cell culture: Bicine is sometimes utilized as a buffering agent in cell culture media, particularly for certain types of cells that require a narrower pH range for optimal growth and viability.
    • Protein purification: Bicine can be used as a component in protein purification buffers to maintain the desired pH during the purification process.
  6. Safety considerations: Bicine is generally considered safe to handle if standard laboratory practices for chemical handling are followed. However, it is always important to review and adhere to specific safety guidelines, including the use of appropriate personal protective equipment (PPE), when working with any chemical compound.

Bicine is commercially available as a powder and is typically dissolved in water to prepare the desired buffer solution at the desired concentration. The pH of a Bicine solution can be adjusted by adding either Bicine or acid/base solutions to increase or decrease the pH, respectively.

 

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