What are the preparation techniques of trishydroxymethylaminomethane hydrochloride?
As a buffer commonly used in biochemical experiments, trishydroxymethylaminomethane hydrochloride has low ionic strength, good buffering effect, and can ensure the survival of proteins in a suitable pH environment, so it is often used to prepare various buffers . Although it is very easy to buy tris hydrochloride in the market, sometimes it is difficult to use the product due to interference from external factors. At this time, it is necessary to fully understand its preparation process in order to solve the problem once and for all. The following is a detailed introduction to its preparation process.
1. Prepare materials
According to the concentration of the required solution and the amount of buffer, fully calculate the required amount, and prepare an appropriate amount of tris (Tris), deionized water, and hydrochloric acid.
1. First of all, the concentration and volume of Tris buffer should be roughly determined. For example, the concentration of saline in Tris buffer may vary. If the substance to be prepared is determined, it can be calculated through the concentration of the buffer solution, and then the required moles of Tris can be obtained.
2. After obtaining the number of moles, multiply the molecular weight of Tris to determine its grams. After the determination is completed, dissolve the Tris powder in deionized water to reach the required volume range.
3. After the required volume is obtained, HCl can be mixed in until the pH meter provides the pH value of the required buffer solution, and then the buffer solution is diluted with water to achieve the required solution volume.
4. After the solution is prepared, it can be stored indoors at room temperature aseptically for several months, and it can be used as soon as possible.
At present, there are many manufacturers of Tris-HCL buffer in the market, but there are not many manufacturers with excellent quality and stable process. As an advantageous supplier of biological buffer, Desheng has continuously improved its technology in recent years, and its products are sold in many regions around the world. There are transactions, widely praised by the industry. Among them, the commonly used buffer products such as Tris, Mops, Hepes, Taps, etc. are in stock, can provide customers with bulk orders, and are equipped with professionals for after-sales service. If you are interested in the products of Desheng Company, please click on the website or call us for details.
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