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CAPSO: A reliable buffer choice for supporting high-pH biochemical experiments.
Latest company news about CAPSO: A reliable buffer choice for supporting high-pH biochemical experiments.

In research and applications of biochemistry, molecular biology, and in vitro diagnostics, maintaining pH stability of the reaction system is a fundamental prerequisite for ensuring the accuracy and reproducibility of experimental results. For this reason, researchers widely use a class of compounds known as Good's buffers, among which CAPSO (3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid, CAS No.: 73463-39-5) has become a commonly used buffer component in experimental systems due to its excellent performance under alkaline conditions.

 

Basic Physicochemical Properties of CAPSO

 

CAPSO is a white crystalline powder, readily soluble in water, with a pKa value of 9.6 at 25°C and an effective buffering range of pH 8.9–10.3. This characteristic makes it particularly suitable for biochemical reactions that need to be carried out in weakly alkaline to moderately alkaline environments. Compared to other traditional buffer systems (such as carbonate or glycine-NaOH), CAPSO has lower ionic interference, good thermal stability, and minimal impact on most enzyme activities, and is therefore widely used in experimental scenarios requiring precise control of the reaction environment.

 

Practical Application in Membrane Protein Extraction

 

Due to their strong hydrophobicity and complex structure, membrane proteins are highly susceptible to denaturation or precipitation during extraction due to environmental changes. Existing technical solutions show that CAPSO can be used to prepare membrane protein lysis buffer. In such systems, CAPSO effectively inhibits drastic fluctuations in local pH during cell lysis and maintains the ionic strength of the solution at near-physiological levels. Furthermore, CAPSO does not readily form strong complexes with metal ions (such as Ca²⁺, Mg²⁺), which helps prevent proteins from forming insoluble complexes by binding to polyvalent cations, thereby improving the recovery rate and functional integrity of the target protein.

 

Supporting Various Key Experimental Techniques

 

In addition to membrane protein research, CAPSO also plays an important role in protein analysis techniques such as Western blotting and immunoprecipitation (IP). In these experiments, a stable pH environment facilitates the specific binding of antibodies to antigens, reducing non-specific background signals. At the same time, in some enzyme-catalyzed reactions that depend on alkaline conditions (such as alkaline phosphatase-labeled detection systems), CAPSO can provide suitable and sustained buffering capacity, improving detection sensitivity and linear range. In the development of in vitro diagnostic reagents, CAPSO is also used to optimize reaction buffer systems, enhancing reagent stability and batch-to-batch consistency.

 

High-quality raw materials ensure experimental reliability.

 

As life science research develops towards greater precision and standardization, the requirements for buffer purity, impurity content, and batch-to-batch consistency are increasing. As a key buffer raw material, the quality of CAPSO directly affects the success rate of downstream experiments and the reliability of data. Therefore, choosing a supplier with a rigorous quality control system and stable supply capacity has become a common consensus among research institutions, diagnostic reagent companies, and biopharmaceutical companies.

 

Hunan Yunbang Biotech Inc. specializes in the research and production of biological buffers and can provide high-purity CAPSO (CAS: 73463-39-5), suitable for various applications including scientific research, in vitro diagnostics, and bioprocessing. The company has a comprehensive quality management system and a professional technical support team, dedicated to providing customers with stable and reliable buffer solutions. If you are looking for a reliable CAPSO supplier, please contact us – we will support your research and industrialization process with professional quality.

Pub Time : 2026-01-26 16:35:32 >> News list
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