|Color:||White||Melting Point:||230 °C|
CAS 7240-90-6 Lab Reagent,
7240-90-6 X-GAL Glycoside,
X-GAL CAS7240-90-6 Glycoside 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
|5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Basic information|
|Synonyms:||X-BETA-D-GAL;X-BETA-D-GALACTOSIDE;X-BETA-GAL;X-GAL READY TO USE;X-GAL;5-BROMO-4-CHLORO-1H-INDOL-3-Y L BETA-D-GALACTOPYRANOSIDE;5-BROMO-4-CHLORO-1H-INDOL-3-Y L BETA-D-GALACTOSIDE-(1,5);5-BROMO-4-CHLORO-3-INDOLYL-B B-D-GALACTOPYRANOSIDE|
|5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Chemical Properties|
|Melting point||230 °C|
|alpha||-64 º (C=1, H2O/DMF (1/1))|
|Boiling point||673.9±55.0 °C(Predicted)|
|density||1.5563 (rough estimate)|
|refractive index||1.6110 (estimate)|
|solubility||Soluble in 100 mM in DMSO.|
|Sensitive||Moisture & Light Sensitive|
|Stability:||Stable. Incompatible with strong oxidizing agents.|
|CAS DataBase Reference||7240-90-6(CAS DataBase Reference)|
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside (X-Gal) is a commonly used substrate in molecular biology and biochemistry to detect the activity of the enzyme β-galactosidase. It is widely used in assays that involve the analysis of gene expression and protein localization.
Here are some key points about X-Gal:
Principle: X-Gal is a colorless substrate that, in the presence of β-galactosidase, is hydrolyzed to yield a blue product. This enzymatic reaction allows the detection of β-galactosidase activity in cells or tissues.
Application: X-Gal is often used in conjunction with the lacZ gene, which encodes β-galactosidase. In molecular biology, researchers commonly employ lacZ as a reporter gene to study gene expression, promoter activity, and protein localization. X-Gal staining can reveal the presence and activity of β-galactosidase, indicating the expression of the lacZ gene.
Color development: When X-Gal is hydrolyzed by β-galactosidase, it yields a blue product called 5,5'-dibromo-4,4'-dichloroindigo. This blue color is visually detectable and allows for qualitative analysis of β-galactosidase activity.
Substrate specificity: X-Gal is specifically cleaved by β-galactosidase and is not hydrolyzed by most other common enzymes, making it a specific and reliable substrate for detecting β-galactosidase activity.
Handling and storage: X-Gal is typically available as a powder that can be dissolved in a suitable solvent, such as dimethylformamide (DMF) or dimethyl sulfoxide (DMSO), to prepare a stock solution. It should be stored at -20°C in a dry and dark environment to maintain its stability.
Safety precautions: As with any chemical reagent, it is important to follow proper safety protocols when handling X-Gal. Avoid direct contact with the skin, eyes, or ingestion, and work in a well-ventilated area.
X-Gal has been widely used in various fields of research, including molecular biology, genetics, and developmental biology, as a valuable tool for visualizing and studying gene expression patterns.
Contact Person: Maggie Ma
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