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Product Details:
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| Name: | Geneticin | CAS: | 108321-42-2 |
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| Form: | Powder | Color: | White To Off-white |
| Storage Temp.: | 2-8°C | ||
| High Light: | 108321-42-2 Geneticin Disulfate Salt,Geneticin Disulfate Salt |
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G418 CAS 108321-42-2 Geneticin Disulfate Salt White To Off White
| Basic information |
| Product Name: | Geneticin |
| CAS: | 108321-42-2 |
| MF: | C20H42N4O14S |
| MW: | 594.63 |
| EINECS: | 600-864-4 |
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| Geneticin Chemical Properties |
| storage temp. | 2-8°C |
| solubility | H2O: 50 mg/mL |
| form | white powder |
| color | white to off-white |
| optical activity | [α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC)) |
| Water Solubility | soluble |
| InChIKey | QNQZPJLBGRQFDD-ZLRXUJDMSA-N |
| Safety Information |
| Hazard Codes | Xn,Xi |
| Risk Statements | 20/21/22-36/37/38-42/43 |
| Safety Statements | 22-24/25-36/37-23-36-26-45 |
| WGK Germany | 3 |
| RTECS | CB9378500 |
| HS Code | 29419090 |
| Geneticin Usage And Synthesis |
| Description | Geneticin (also known as G418, G418 sulfate) is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. Geneticin blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells and is commonly used in laboratory research to select genetically engineered cells. Resistance to Geneticin is conferred by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APH 3'II. |
| Chemical Properties | white to off-white powder |
| Uses | Geneticin is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL. It is also used to membrane-permeant allosteric effector of hemoglobin therapeutic in cardiovascular diseases and cancer. |
| Application | Geneticin's most common application is in molecular biology as a selection agent. Geneticin(G418 sulfate) is toxic to bacteria, yeast, protozoa, helminths, and mammalian cells. Resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. G418 is water soluble and can be stored at room temperature for as long as 1 year. Aqueous solutions should be stored frozen. The amount of G418 required for selection will vary with each cell type and growth cycle. Although cells that are multiplying will be affected sooner than those that are not, cells that are in log phase will still require 3 to 7 days for selection. In general, the amount of G418 required for selection of mammalian cells is 400 mg/ml and 200 mg/ml for maintenance. |
Contact Person: Maggie Ma
Tel: +0086 188 7414 9531
