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Ethidium bromide powder CAS 1239-45-8 Biological Dyes

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Ethidium bromide powder CAS 1239-45-8 Biological Dyes

Ethidium bromide powder CAS 1239-45-8 Biological Dyes
Ethidium bromide powder CAS 1239-45-8 Biological Dyes Ethidium bromide powder CAS 1239-45-8 Biological Dyes Ethidium bromide powder CAS 1239-45-8 Biological Dyes Ethidium bromide powder CAS 1239-45-8 Biological Dyes

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Product Details:
Place of Origin: China
Brand Name: YunbangPharm
Certification: ISO9001
Model Number: YB006
Payment & Shipping Terms:
Minimum Order Quantity: Negotiable
Price: Negotiable
Packaging Details: 100g,200g,1kg/Bottle, 5-25kg/Drum
Delivery Time: 3-5 working days
Payment Terms: L/C, D/A, D/P, T/T, Western Union

Ethidium bromide powder CAS 1239-45-8 Biological Dyes

Description
Product Name: Ethidium Bromide CAS NO: 1239-45-8
Form: Powder Sample: Available
Package: 10g,50g,100g,1kg Formula: C21H20BrN3
Solubility: H2O: 10 Mg/mL, Opaque, Strongly Red Odour: Odorless Solid

Ethidium bromide powder CAS 1239-45-8 Biological Dyes

 

Product Name:

Ethidium bromide solution

Synonyms:

3,8-diaMino-5-ethyl-6-phenylphenanthridin-5-iuM broMide;EthidiuM broMide dropper bottle,5Ml;2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide, 1% aqueous solution;Ethidinium bromide, 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide;EthidiuM BroMide, OMniPur(R);EthidiuM broMide, pure, 95% 1GR;EthidiuM broMide, pure, 95% 5GR;Dromilac

CAS:

1239-45-8

MF:

C21H20BrN3

MW:

394.32

EINECS:

214-984-6

Product Categories:

marker;Amines;Aromatics;Intermediates & Fine Chemicals;Pharmaceuticals;Phenanthridine;Purines

Mol File:

1239-45-8.mol

Ethidium bromide Structure

 

Melting point

260-262 °C (dec.)(lit.)

density

1.3739 (rough estimate)

refractive index

1.6700 (estimate)

Fp

>100°C

storage temp.

2-8°C

solubility

H2O: 10 mg/mL, opaque, strongly red

form

powder

color

Red to dark purple

Odor

Odorless solid

Water Solubility

40 g/L (25 ºC)

λmax

518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm

Merck

14,4731

BRN

3642536

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification

Major Application

Electroluminescent displays;photoresists

CAS DataBase Reference

1239-45-8(CAS DataBase Reference)

EPA Substance Registry System

Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1239-45-8)

 

 

Application:

Ethidium bromide solution has been used:
  • as a stain to visualize U937 cells to assess cell viability
  • to detect polymerase chain reaction products
  • in agarose gel electrophoresis based gel retardation assay
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Biochem/physiol Actions:

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
 

Reconstitution:

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis
 
 
Ethidium bromide powder CAS 1239-45-8 Biological Dyes 1

 

 

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