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HEPES Biochemical Reagents CAS 7365-45-9 Molecular Biology

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HEPES Biochemical Reagents CAS 7365-45-9 Molecular Biology

HEPES Biochemical Reagents CAS 7365-45-9 Molecular Biology
HEPES Biochemical Reagents CAS 7365-45-9 Molecular Biology HEPES Biochemical Reagents CAS 7365-45-9 Molecular Biology

Large Image :  HEPES Biochemical Reagents CAS 7365-45-9 Molecular Biology

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Place of Origin: China
Brand Name: YunbangPharm
Certification: ISO9001
Model Number: YB015
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Minimum Order Quantity: Negotiable
Price: Negotiable
Packaging Details: 1kg/Bottle, 5-25kg/Drum
Delivery Time: 5-8 working days
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Supply Ability: 200kg

HEPES Biochemical Reagents CAS 7365-45-9 Molecular Biology

Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic Acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic Acid) Cas No: 7365-45-9
Application: Molecular Biology Purity: 99%
Classification: Biological Buffer Form: Crystal Powder
Color: White Samples: Available
Package: 1kg/5kg/25kg Transport: Courier/Air/Sea
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HEPES Biochemical Reagents


ISO9001 Biochemical Reagents


CAS 7365-45-9


HEPES CAS 7365-45-9 Biological Buffers Molecular Biology Grade (free acid)


HEPES Basic information
Product Name: HEPES
Synonyms: HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE;HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+%;N-(2-HYDROXYETHYL)-PIPERAZINE-N'-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID));Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz;1-HeptaneSulfonicAcidSod.SaltGr;N-(2-Hydroxyethyl)Piperazine-N'-(3-EthanesulfonicAcid);N-(2-Hydroxyethyl)Piperazine-N'-(2-EthanesulfonicAcid)(Hepes);Hepes,LowSodium,FreeAcid
CAS: 7365-45-9
MF: C8H18N2O4S
MW: 238.3
EINECS: 230-907-9
Product Categories: Disinfectants;Biochemistry;Good's Buffers;Buffer;ReagentsBiological Buffers;GMP;AlphabeticalMolecular Biology;Biological Buffers;BioUltra Buffers;BioUltraMolecular Biology;DNA&RNA Purification;Molecular Biology Reagents;Zwitterionic;VX:15689727968
Mol File: 7365-45-9.mol
HEPES Structure
HEPES Chemical Properties
Melting point 234-238 °C
density 1.07 g/mL at 20 °C
refractive index n20/D 1.339
storage temp. 2-8°C
solubility H2O: 1 M at 20 °C, clear, colorless
form powder
color White
PH 5.0-6.5 (1Maqueous solution)
PH Range 7.0 - 7.6
pka 7.5(at 25℃)
Water Solubility Soluble
λmax λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
Merck 14,4654
BRN 883043
Stability: Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
HEPES Usage And Synthesis
Description HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Chemical Properties White crystalline powder
Uses Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
Uses Biological buffer.
Uses HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.
Definition ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
General Description HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
Biological Activity HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
Biological Activity Multi purpose buffer used in biological research.
Purification Methods Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]


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