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Product Details:
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| Storage Temp.: | -20°C | Form: | Powder |
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| Color: | White | Melting Point: | 230 °C |
| CAS: | 7240-90-6 | ||
| High Light: | CAS 7240-90-6 Lab Reagent,7240-90-6 X-GAL Glycoside,5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactoside |
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X-GAL CAS7240-90-6 Glycoside 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
| 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Basic information |
| Product Name: | 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside |
| Synonyms: | X-BETA-D-GAL;X-BETA-D-GALACTOSIDE;X-BETA-GAL;X-GAL READY TO USE;X-GAL;5-BROMO-4-CHLORO-1H-INDOL-3-Y L BETA-D-GALACTOPYRANOSIDE;5-BROMO-4-CHLORO-1H-INDOL-3-Y L BETA-D-GALACTOSIDE-(1,5);5-BROMO-4-CHLORO-3-INDOLYL-B B-D-GALACTOPYRANOSIDE |
| CAS: | 7240-90-6 |
| MF: | C14H15BrClNO6 |
| MW: | 408.63 |
| EINECS: | 230-640-8 |
| Mol File: | 7240-90-6.mol |
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| 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Chemical Properties |
| Melting point | 230 °C |
| alpha | -64 º (C=1, H2O/DMF (1/1)) |
| Boiling point | 673.9±55.0 °C(Predicted) |
| density | 1.5563 (rough estimate) |
| refractive index | 1.6110 (estimate) |
| storage temp. | -20°C |
| solubility | Soluble in 100 mM in DMSO. |
| pka | 12.74±0.70(Predicted) |
| form | Powder |
| color | White |
| Odor | Odorless |
| Sensitive | Moisture & Light Sensitive |
| Merck | 14,10074 |
| BRN | 1402009 |
| Stability: | Stable. Incompatible with strong oxidizing agents. |
| InChIKey | OPIFSICVWOWJMJ-AEOCFKNESA-N |
| CAS DataBase Reference | 7240-90-6(CAS DataBase Reference) |
| Safety Information |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 24/25-37/39-26 |
| WGK Germany | 3 |
| F | 8-10-21 |
| HazardClass | IRRITANT |
| HS Code | 29389090 |
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside (X-Gal) is a commonly used substrate in molecular biology and biochemistry to detect the activity of the enzyme β-galactosidase. It is widely used in assays that involve the analysis of gene expression and protein localization.
Here are some key points about X-Gal:
Principle: X-Gal is a colorless substrate that, in the presence of β-galactosidase, is hydrolyzed to yield a blue product. This enzymatic reaction allows the detection of β-galactosidase activity in cells or tissues.
Application: X-Gal is often used in conjunction with the lacZ gene, which encodes β-galactosidase. In molecular biology, researchers commonly employ lacZ as a reporter gene to study gene expression, promoter activity, and protein localization. X-Gal staining can reveal the presence and activity of β-galactosidase, indicating the expression of the lacZ gene.
Color development: When X-Gal is hydrolyzed by β-galactosidase, it yields a blue product called 5,5'-dibromo-4,4'-dichloroindigo. This blue color is visually detectable and allows for qualitative analysis of β-galactosidase activity.
Substrate specificity: X-Gal is specifically cleaved by β-galactosidase and is not hydrolyzed by most other common enzymes, making it a specific and reliable substrate for detecting β-galactosidase activity.
Handling and storage: X-Gal is typically available as a powder that can be dissolved in a suitable solvent, such as dimethylformamide (DMF) or dimethyl sulfoxide (DMSO), to prepare a stock solution. It should be stored at -20°C in a dry and dark environment to maintain its stability.
Safety precautions: As with any chemical reagent, it is important to follow proper safety protocols when handling X-Gal. Avoid direct contact with the skin, eyes, or ingestion, and work in a well-ventilated area.
X-Gal has been widely used in various fields of research, including molecular biology, genetics, and developmental biology, as a valuable tool for visualizing and studying gene expression patterns.
Contact Person: Maggie Ma
Tel: +0086 188 7414 9531
