|Name:||3-(N-Morpholino)propanesulfonic Acid, 4-Morpholinepropanesulfonic Acid||Cas No:||1132-61-2|
MOPS Buffer CAS 1132-61-2 Biological Buffers 3-Morpholinopropanesulfonic acid Free Acid
|3-Morpholinopropanesulfonic acid Basic information|
|Product Name:||3-Morpholinopropanesulfonic acid|
|Synonyms:||MOPS;MOPS, CONCENTRATE SOLUTION;MORPHOLINOPROPANE SULFONIC ACID;TIMTEC-BB SBB009133;4-MORPHOLINEPROPANESULFONIC ACID;4-(MORPHOLINOPROPANE SULFONIC ACID);3-MORPHOLINOPROPANESULFONIC ACID;3-MORPHOLINOPROPANESULPHONIC ACID|
|Product Categories:||Imidazoles;Buffer;Miscellaneous Biochemicals;Organic acids;Biochemistry;Good's Buffers|
|3-Morpholinopropanesulfonic acid Chemical Properties|
|Melting point||277-282 °C|
|density||1.3168 (rough estimate)|
|refractive index||1.6370 (estimate)|
|storage temp.||room temp|
|solubility||H2O: 1 M at 20 °C, clear|
|PH||2.5-4.0 (25℃, 1M in H2O)|
|PH Range||6.5 - 7.9|
|Water Solubility||1000 g/L (20 ºC)|
|λmax||λ: 260 nm Amax: 0.020
λ: 280 nm Amax: 0.015
|Stability:||Stable. Incompatible with strong bases, strong oxidizing agents.|
|3-Morpholinopropanesulfonic acid Usage And Synthesis|
|Description||MOPS (3-morpholinopropanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES. Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.It is used as a synthetic buffering agent below pH 7.5.|
|application||MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis. Usage above 20 mM in mammalian cell culture work is not recommended. MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.|
|Reference||P. H. Quail, D. Marme, E. Schäfer, Particle-bound phytochrome from maize and pumpkin, Nature New Biology, 1973, vol. 245, pp. 189-191|
|Chemical Properties||White/clear crystalline powder|
|Uses||3-(N-Morpholino)propanesulfonic acid or MOPS due to its inert nature is a preferred and widely used buffer in many biochemical studies.
MOPS has been used as:
a cell culture additive component in lentiviral particle production.
as a buffering agent in microbial growth medium and nuclei extraction buffer.
as a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum.
as a buffer in capillary-zone electrophoresis to test performance.
for the dilution of proteins from algal samples.
|Uses||MOPS acts as a multi-purpose buffering agent used in various biological research.|
|Uses||MOPS has been used as:
|Definition||ChEBI: 3-(N-morpholino)propanesulfonic acid is a Good's buffer substance, pKa = 7.2 at 20 ℃. It is a member of morpholines, a MOPS and an organosulfonic acid. It is a conjugate acid of a 3-(N-morpholino)propanesulfonate. It is a tautomer of a 3-(N-morpholiniumyl)propanesulfonate.|
|General Description||3-(N-Morpholino)propane sulfonic acid (MOPS) is an N-substituted amino sulfonic acid with a morpholinic ring. MOPS is capable of buffering within a pH range of 6.5-7.9. MOPS is widely used in biological and biochemical studies due to its inert properties. It does not interact with any metal ions in solutions and has significant metal-buffer stability especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions. MOPS buffer maintains the pH of the mammalian cell culture medium. MOPS functions to maintain pH in denaturing gel electrophoresis of RNA. MOPS can modify lipid interactions and influence the thickness and barrier properties of membranes. MOPS interacts with bovine serum albumin and stabilizes the protein. Hydrogen peroxide oxidizes MOPS slowly to N-oxide form.|
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