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Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis

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Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis

Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis
Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis

Large Image :  Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis

Product Details:
Place of Origin: China
Brand Name: YunbangPharm
Certification: ISO9001
Model Number: YB007
Payment & Shipping Terms:
Minimum Order Quantity: Negotiable
Price: Negotiable
Packaging Details: 1kg/Bottle, 5-25kg/Drum
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T, Western Union
Supply Ability: 200kg

Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis

Description
Name: N-[Tris(hydroxymethyl)methyl]glycine Cas No: 5704-04-1
Application: Molecular Biology/ Electrophoresis Buffer Purity: 99%
Classification: Biological Buffer Form: Powder
Color: White Samples: Available
Package: 1kg/5kg/25kg Transport: Courier/Air/Sea
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Electrophoresis Tris Tricine Buffer

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Biological Tris Tricine Buffer

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CAS 5704-04-1

 

Tricine CAS 5704-04-1 Biological Buffer 99% Good's Buffer Electrophoresis buffer

 

 

Tricine Basic information
Descriptioin Reference
Product Name: Tricine
Synonyms: LABOTEST-BB LT00454966;n-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-glycin;TRICINE;TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE;TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE;TRIS-HCL BUFFER;TRIClNE;E-Z 96 SIZE SELECT-IT (1 X 96)
CAS: 5704-04-1
MF: C6H13NO5
MW: 179.17
EINECS: 227-193-6
Product Categories: Biochemistry;Good's Buffers;Buffer
Mol File: 5704-04-1.mol
Tricine Structure
 
Tricine Chemical Properties
Melting point 186-188 °C (dec.)
Boiling point 311.69°C (rough estimate)
density 1.05 g/mL at 20 °C
refractive index 1.4240 (estimate)
storage temp. Keep in dark place,Inert atmosphere,Room temperature
solubility H2O: 4 M at 20 °C, clear, colorless
form crystalline
color clear colorless (40 % (w/w) in H2O) solution
Odor Odorless
PH 4.0-6.0 (1M in H2O)
PH Range 7.4 - 8.8
pka 8.1(at 25℃)
Water Solubility soluble
Merck 14,9651
BRN 1937804
Stability: Stable.
CAS DataBase Reference 5704-04-1(CAS DataBase Reference)

 

Tricine Usage And Synthesis
Descriptioin Tricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.3
Reference
  1. S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
  2. H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379
Chemical Properties White/clear crystalline powder
Uses Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets.
A zwitterionic buffer useful in the pH range of 7.4-8.8. Has a pKa 8.05 at 25°C.
A zwitterionic amino acid commonly useful in the pH range of 7.4-8.8. Has a pKa 8.05 at 25°C.
Uses Tricine is a commonly used electrophoresis buffer.
Uses Trizma? Buffer substance has been used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).
General Description Trizma? and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.
Biological Activity Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.
Purification Methods Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]

 

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General description

Tricine is the most frequently used electrophoresis buffer. It is also useful for the resuspension of cell pellets. Tricine functions as a trailing ion and aids the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. It has a lower negative charge than glycine, which helps it migrate faster. Tricine has a high ionic strength that allows increased ion movement and less protein movement. This in turn leads to the separation of low molecular weight proteins in lower percent acrylamide gels.

Application

Tricine has been used:
  • to prevent precipitation of salts during autoclaving of Emiliania huxleyi cultures
  • as a component of buffer A for the homogenization of samples like Caenorhabditis elegans, Drosophila, and plants
  • as a component of fresh assay buffer to measure serum melatonin by radioimmunoassay
Buffer component for separation of low molecular weight peptides.
 
Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis 1Tris Tricine Buffer 99% Biological Good'S Buffer CAS 5704-04-1 Electrophoresis 2

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